This review focuses on the deamidation of asparagine in monoclonal antibodies. one of the well-known degradation pathways. The importance of close monitoring of deamidation from early developability assessment to in vivo studies is discussed consequentially. The application of chromatographic and electrophoretic instruments for the separation of deamidated variants is described. https://www.rxkaitori.com/product-category/led-emergency-twin-spot/